A control sample of unruptured Schizosaccharomyces pombe yeast.
Schizosaccharomyces pombe after one pass at 28000 psi/193MPa through
the EmulsiFlex-C160. Only cell fragments remain.
|
Cell
Rupture
Top
| Strain: |
Schizosaccharomyces pombe |
| Machine: |
EmulsiFlex-C160 |
| Pressure: |
28000psi/193MPa |
| Sample |
cfu/ml |
% viable |
% NOT viable |
| control |
5.1 x 107 |
~100 |
~0 |
| 1 pass |
5.0 x 103 |
0.01 |
99.99 |
| Strain: |
E. coli (BL21) |
| Machine: |
EmulsiFlex-C160 |
| Pressure: |
20000psi/138MPa |
| Sample |
cfu/ml |
% viable |
% NOT viable |
| control |
4.06 x 109 |
~100 |
~0 |
| 1 pass |
1.77 x 107 |
0.44 |
99.56 |
| 2 passes |
1.70 x 106 |
0.04 |
99.96 |
| 3 passes |
2.4 x 104 |
5.9 x 10-4 |
~100 |
|
An
unprocessed dispersion with a starting size range of 10-100microns (600X
magnification).
Processed
sample of uniform, 1micron particles (100X magnification).
|
Dispersions
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A whole range of dispersions can be run through EmulsiFlex high pressure
homogenizers to reduce particle size as well as make the size distribution
uniform. EmulsiFlex homogenizers are involved in the processing of
dispersions such as drug dispersions, herb extracts, industrial coatings
and pigments.
Shown is a sample of a dry crystalline (amorphous) powder run through
an EmulsiFlex homogenizer at 25000psi/173MPa. |
Liposomes prepared using the EmulsiFlex high pressure homogenizer, extruder
or both in combination. Combined homogenization yields 113.7nm in
one pass and 89.9nm in two passes.
An example of uniform, unilamellar liposomes.
|
Liposomes
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AVESTIN's high pressure homogenizers are used all over the world
for liposome research and production. Only EmulsiFlex homogenizers
offer the combination of high pressure homogenization, high pressure membrane
filtration or a combined, homogenization/filtration process.
|
Pharmaceutical drug emulsion 'A' before processing. The starting
particle size is large (5-10microns) and the distribution wide.
Pharmaceutical
emulsion 'A' after processing at 18000psi/124MPa. Note the dramatic
reduction in size (52nm) and the narrower distribution.
|
Emulsions
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| Oil in water emulsion |
| Sample |
Pressure
(psi) |
Diameter
(nm) |
| unprocessed |
- |
656 |
| 1 pass |
16,000 |
77 |
| 3 passes |
16,000 |
59 |
| 5 passes |
16,000 |
42 |
| Pharmaceutical Emulsion |
| Sample |
Homogenizing
Pressure (psi) |
Mean Droplet
Diameter (nm) |
| control |
- |
3,500 |
| 1 pass |
20,000 |
590 |
| 3 passes |
20,000 |
185 |
| 5 passes |
20,000 |
140 |
|